2017-8-11 16:0:32025-04-08 01:36:12
description
penicillin-binding protein 2C
class A penicillin-binding protein 2C
locus
BSU10110
BSU_10110
geneLength
2142
2145
function
bifunctional glucosyltransferase/ transpeptidase
bifunctional glucosyltransferase/ transpeptidase, synthesis of spore peptidoglycan
ec
outlinks
bsu
BSU10110
BSU_10110
Gene
Coordinates
1,083,851 → 1,085,995
1,083,851 1,085,995
The protein
[SW|Localization]
cell membrane (according to Swiss-Prot), prespore [Pubmed|15758244]
cell membrane, the major part is exposed to the outside (according to UniProt)
prespore [Pubmed|15758244]
during vegetative growth: septal, low flourescence at periphery [pubmed|14731276]
References
Gene
Phenotypes of a mutant
a strain lacking all four class A [SW|penicillin-binding proteins] ([[gene|ponA]] [[gene|pbpD]] [[gene|pbpF]] [[gene|pbpG]]) is severely inhibited for L-form switching in the presence of D-cycloserine [pubmed|29456081]
The protein
Catalyzed reaction/ biological activity
synthesis of spore peptidoglycan (with [[protein|PbpG]]) [pubmed|11567005]
[GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n)-diphospho-di-trans,octa-cis-undecaprenol + β-D-GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)-diphospho-di-trans,octa-cis-undecaprenol = [GlcNAc-(1→4)-Mur2Ac(oyl-L-Ala-γ-D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-diphospho-di-trans-octa-cis-undecaprenol + di-trans,octa-cis-undecaprenyl diphosphate + H+ (according to UniProt)
Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine (according to UniProt)
The protein
Protein family
N-terminal part: [SW|glycosyltransferase 51 family] (according to UniProt)
C-terminal part: [SW|transpeptidase family] (according to UniProt)
The protein
Paralogous protein(s)
[[protein|PbpD]], [[protein|PbpG]], [[protein|PonA]]
The protein
Structure
[PDB|2OLU] (from Staphylococcus aureus, aa 40-610, 32% identity) [pubmed|17347437]
Biological materials
Mutant
BKE10110 ([[gene|pbpF]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACTCACCTCGCCTTT, downstream forward: _UP4_TAATGGAATTCGGCGATTTT
BKK10110 ([[gene|pbpF]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGAACTCACCTCGCCTTT, downstream forward: _UP4_TAATGGAATTCGGCGATTTT
Biological materials
GFP fusion
2084 [[gene|trpC]]2 [[gene|pbpF]]::pSG1491 (cat Pxyl-gfpa-[[gene|pbpF]]1-335) [pubmed|14731276], available in [SW|Dirk Jan Scheffers]' lab and in the [http://bgsc.org BGSC]